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              各類型色譜柱|菲羅門色譜柱|手性柱|氣相柱|SPE|高效液相色譜柱

              Solid-Core Mixed-Mode Stationary Phases

               

              SIELC Technologies has developed mixed-mode Columns based on solid-core-porous-shell particles. This separation media combines the efficiency of solid-core technology with the unique selectivity of Ion-Exchange (IE), reverse phase (RP), surface chemistry and ion-exchange HILIC surface.

              Combination of solid core particles and IE with RP or HILIC chemistry offers:

              • Fast separation
              • Working pressure within limits of conventional HPLC
              • Unique selectivity profile
              • Retention of compounds difficult to retain by RP chromatography
              • Increase column loading capacity
              • Adjustment of selectivity by mobile phase pH, ion strength, and organic concentration and composition

               

              Columns available in Solid-Core format with 2.7 μm particles:

              Column Ion-exchange Separation mode
              Primesep 100 Cation exchange RP
              Primesep SB Anion exchange RP
              Primesep S Cation exchange HILIC

               

              One disadvantage of the solid core support in HPLC is lower capacity when you compare it to regular through porous material. However, the mixed-mode materials demonstrated much higher loading capacity compared to regular RP phases (Nicola H. Davies, Melvin R. Euerby and David V. McCalley, Journal of Chromatography A, Volume 1138, Issues 1-2, 5 January 2007,  65-72). Thus, the mixed-mode surface chemistry compensates for loss of loading capacity of partially non-porous particles.

              Mixed mode columns demonstrate 2D characteristics with independent adjustment of degree of retention based on ionic and hydrophobic mechanism
              Reverse phase mechanism controls by amount of organic modifier in the mobile phase
              Ion-exchange mechanism controls by ion strength and pH of the mobile phase
              This property allows to resolve complex mixtures with polar, hydrophobic, ionic, and neutral compounds

               

              Some separation examples:

               

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